Methods:
I will use canopy cranes, tree pruners, or shotgun to get upper canopy limb clippings of mature trees of a variety of species. Limbs will be immediately cut, placed in water, and taken to a central location. There, one leaf per limb will be analyzed. Analyses performed will be A/Ci curves and dark respiration measurements. The data will be combined with data from a global set of sites to explore variation in the temperature acclimation potential of Rubisco carboxylation (Vcmax), Ribulos-1,5-bisphosphate regeneration (Jmax), and dark respiration (Rd) among species and biomes. These data will be used to create PFT-specific formulations for acclimation that will be included into multiple land surface models. These models will then be evaluated in terms of their ability to reproduce flux tower data, including data from sites used to parameterize the formulations.