With the metagenomic revolution the mycological community has understood that most of the fungi in the environment have never been named. One of the major challenges is that these organisms are microscopic and are unculturable. In the last few decades, molecular systematics has revealed that the aquatic fungi are incredibly diverse at both the species and phylum level. These discoveries have been fueled by culture-independent methods such as metabarcoding and metagenomics. Our lab has been exploring the diversity of aquatic fungi using single cell genomics methods that we have developed to explore fungal diversity. The aquatic fungi are particularly rich at UMBS due to the wealth of data on aquatic fungi determined by Frederick Sparrow and his students. In this project we will sample various aquatic and soil habitats for these fungi and using a number of techniques describe and integrate them into a modern classification system.