Methods:
There will be four different treatments in this study: Control, chemical lesion, visual lesion, and mechanical lesion. Within each treatment there will be three different sizes of catfish that crayfish will encounter: smaller than the crayfish, same size as the crayfish and larger than the crayfish. Each treatment will have 30 trials, 10 trials for each size of catfish. The total number of trials for this study will be 120. A naïve crayfish will be used for each trial to prevent any learning by the crayfish. The trials will last for 15 minutes each in an experimental arena which will be an open flow system. The behavior of the crayfish will be compared to a behavioral ethogram which will give each behavior a numerical value. The numerical values will be used in statistical analysis to compare between the treatments of lesions and size differences in catfish.