Methods:
The Field Mammalogy course includes small mammal surveys as part of its curriculum. We are collaborating with Jessica Light, the course instructor, to have the students include fecal sampling as part of their protocols in areas where white footed mice are particularly common.
At each capture, we will take a fecal sample, and collect other standard data to assess body condition. Specifically, students will record the species, sex, weight, length, and reproductive condition of each individual to pair with the fecal sample data. Half of each fecal sample will be used to quantify gastrointestinal worm burden and stress levels and the other half will be submitted to Jonah Ventures (Boulder, CO) for meta-barcoding to identify the species and relative proportions of plants, arthropods, and fungi consumed by the mice. Meta-barcoding will also be used to verify the identities of the gastrointestinal parasites.
In 2025, we would like to experimentally test how infection burden of helminth parasites influences the health and nutrition of white-footed mice (Peromyscus leucopus). The samples collected during 2024 with the Field Mammalogy students will provide critical pilot data on variation in parasite burden and host diet that will be used to inform our field activities the following year.