Methods:
Water use efficiency (carbon fixed/water lost) will be measured in leaf samples from plants in the Indian River field site (4 species, 10 replicates) to measure species WUE in natural field conditions. It will also be measured in a subset of transplants in the field and common garden experiment to ensure that plants in my experimental setups are mimicking field conditions. I will use a LI-COR (either the LI-COR 6400 owned by the Nadelhoffer lab or one of the BART/UMBS machines) to do these measurements, which simultaneously measures photosynthesis and transpiration. Soil moisture levels in the field and common garden will also be quantified continuously over the field season using capacitance moisture probes (Decagon).