Methods:
Three types of environmental stimuli characteristics within aquatic ecosystems: visual, chemical, and mechanical signals. An Acoustic Doppler Velocimeter (ADV) will be used to measure hydrodynamic characteristics such as turbulence at 25 Hz. An Epsilon electrochemical detection system, a carbon fiber, graphite-epoxy style micro electrode, and dopamine, will be used to measure chemical signals at a rate of 10 Hz. Video cameras will be used to measure luminance at different spectral frequencies
We will chose different natural aquatic habitats because the physical differences in shading, flow, and substrate construct different sensory signals. Sampling of environmental stimuli will take place in both a river and a lake ecosystem because the hydrodynamic differences that occur across these systems alter the structure of chemical signals. In addition, different sections of the lake and river will be chosen based on shading characteristics (altering light intensity) and depth (altering color composition). Finally, different substrates will be chosen due to the different acoustical reflective properties of these substrates.
We will mimic a food source using standard procedures that include fish gelatin and will mimic different naturally shelters The sensory stimuli will be quantified in a 40 cm x 30 cm grid surrounding the focal stimuli (food or shelter) .Within this grid, sample points will be place 10 cm apart in both the x and y direction and 5 sample heights separated by 2 cm in the z direction. There will be a total of 45 sample points.
Data analysis will include analyzing velocity, chemical structures of chemical plumes ,as well as spectral frequencies of light