1. Groundwater Sampling In order to adequately sample the groundwater both in chemical and physical properties, a set of piezometers and seepage meters will be installed along a 50-meter study site equally distributed on either side of Honeysuckle Creek. A series of six shallow onshore piezometers are located along a transect. Three additional onshore piezometers, further offshore than the transect, were installed in order to further quantify groundwater flow. A second row of piezometers is located just offshore in Burt Lake. Approximately 15 meters offshore, rows of seepage meters line the 50-meter study site. The most shallow row has seepage meters placed every 3 meters apart, while the next and deeper row have meters placed 10 meters apart. The next row is deeper still and has seepage meters every 12.5 meters. The last and deepest row also has meters every 12.5 meters. During a two-week period, three mass sampling dates were completed in which the two six-piezometer rows, and all of the seepage meters where used to collect a water sample. For chemical analysis, groundwater was pumped from a tube from the piezometer until as much of the sediment as possible was flushed from the system. To collect physical data, all onshore and offshore piezometers were used to record water table height. A baggie with a known amount of degassed liquid was first weighed and then attached to each seepage meter. Time was recorded with a stopwatch while the baggie filled. Once full, the baggie was removed from the seepage meter and weighed to calculate seepage rate. At each site three 40 mL bottles were filled either directly from the tubing in the piezometers or from the seepage sample baggie. Due to time constraints on processing, at randomized meters along each row, one extra 40 mL bottle was filled for analysis of MeHg. For each sampling date replicates were taken of each piezometer and randomized seepage meters in each row. During this two-week period, two rain events were captured following the same sampling procedures with sampling times of within 18 hours and 32 hours of the rain event. Independent variables of temperature, conductivity, and pH were taken of the groundwater at each sampling location using water quality probes. 2. Surfacewater Sampling A Parshall flume will measure stage and discharge where Honeysuckle Creek meets Burt Lake. Grab samples are taken at one site along the stream. These were taken following the same timing of the groundwater samples, with replicates taken at each sample time. Lake water samples were also collected at each side of the study site following the same sample schedule. At each lake and stream sampling event, four 40 mL sample bottles were filled. Independent variables of temperature, conductivity, and pH were taken of the of surface and lake water at each sampling location using water quality probes. 3. Water analysis Each sample will be transported to prepared and labeled glassware. For all analysis these are amber 40 mL bottles that have been soaked in 10% HCl overnight and triple rinsed with MilliQ water. In the field, the bottles are triple rinsed with the native water before filling. The samples are then preserved with 2 mL HCl. Analysis of Hg and MeHg was completed at University of Michigan, Ann Arbor using a Brooks Rand MERX following method EPA 1631 (EPA, 2002) for Hg and EPA 1630 (EPA, 1998) for MeHg. TOC and DOC analysis was completed at University of Michigan Biological Station using an Aurora 1030W TOC Analyzer, in which roughly twenty manufactured blanks are run with each batch of about 75 samples.