Methods:
This experiment will be a 2 x 3 design with the first factor being predator presence (no predator or predator) and the second factor being prey species (Orconectes virilis, Orconectes rusticus, and Orconectes propinquus). There will be 6 different treatments in this study: Orconectes virilis with no predator, Orconectes virilis with a predator, Orconectes rusticus with no predator, Orconectes rusticus with a predator, Orconectes propinquus with no predator and Orconectes propinquus with a predator. Each treatment will have 10 trials, with a total number of 60 trials for the study. A naïve crayfish will be used for each trial to prevent any learning by the crayfish. The trials will last for 4 hours each. Within each testing arena there will be a shelter and food source for the crayfish. Each trial will be videotaped and time budgets will be calculated to for how much time crayfish spend in the different areas of the prey section: shelter opening, shelter, free, and food areas. The amount of time spent performing these behaviors will be used in statistical analysis to compare between the predator presence and the species.
This study will also investigate the differences in flicking behavior of the crayfish to the presence and absence of the predator odor. The experiment will also be a 2x3 design with the first factor being predator presence (no predator or predator) and the second factor being prey species (Orconectes virilis, Orconectes rusticus, and Orconectes propinquus). There will be 6 different treatments in this study: Orconectes virilis with no predator, Orconectes virilis with a predator, Orconectes rusticus with no predator, Orconectes rusticus with a predator, Orconectes propinquus with no predator, and Orconectes propinquus with a predator. Each treatment will have 10 trials, with a total number of 60 trials for the study. A naïve crayfish will be used for each trial to prevent any learning by the crayfish. All trials will last for 15 minutes and will be video recorded. The videos will be analyzed to for the number of antennule flicks of each crayfish.