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Investigating environmental attributes that influence bumble bee pathogen infections

Project Abstract: 
Bumble Bees (Bombus) are threatened by a number of stressors including land use change, pesticides, and pathogens. The latter is thought to be a leading factor of their decline. In Michigan, seven species are listed as species of conservation concern in the state. Similarly, the federally endangered Bombus affinis and five other species are recently reported to be extirpated from the state. Several factors, such as landscape composition, floral resource quality, and distance from commercial colonies have been shown to influence pathogen transmission; however there are gaps in our understanding of how the surrounding landscape may mediate local environmental effects on pathogen infections in bumble bees. To investigate these unknowns, two species of bumble bee workers will be collected from UMBS sites for pathogen analysis. Broad plant and bumble bee community surveys will be completed in conjunction with pathogen collections. This project will add to ongoing bumble bee pathogen research by expanding into Northern Michigan. The addition of samples from habitats will broaden our knowledge of the distribution and prevalence of bumble bee pathogens across longitudinal ranges, particularly in Michigan, where to our knowledge no formal pathogen survey has been completed for wild bumble bees foraging in natural areas.
Investigators: 
Status of Research Project: 
Years Active: 
2024
Research sites: 
Methods: 
Monthly plant and bumble bee community surveys will be conducted from June-September 2024. Surveys will be conducted across a variety of habitats at UMBS sites. Surveys and rapid habitat assessments will follow adapted protocols described by the Xerces Society’s Bumble Bee Atlas program. This includes an inventory of all flowering plant species and non-lethal photography of captured bumble bees. All associated floral visits of captured bumble bees will also be recorded. Bumble bee collections for pathogen screening will target two species: B. impatiens and B. griseocollis and will occur during and after formal community surveys. These species are not of conservation concern, abundant across the state, and belong to two separate subgenera making a possibly interesting comparison between the two. We will aim to collect 20 workers from both species with a minimum of 15 at each site (N=40). Individuals will be transported in coolers and stored in -20° freezers until they are transported back to the Jamieson Lab. Pathogen screening will later investigate infection of Crithidia bombi, Variamorpha bombi, and conopid fly (Conopidae, Diptera) parasitism through microscopy and molecular analysis.
Funding agency: 
USFWS