Methods:
Monthly plant and bumble bee community surveys will be conducted from June-September 2024. Surveys will be conducted across a variety of habitats at UMBS sites. Surveys and rapid habitat assessments will follow adapted protocols described by the Xerces Society’s Bumble Bee Atlas program. This includes an inventory of all flowering plant species and non-lethal photography of captured bumble bees. All associated floral visits of captured bumble bees will also be recorded. Bumble bee collections for pathogen screening will target two species: B. impatiens and B. griseocollis and will occur during and after formal community surveys. These species are not of conservation concern, abundant across the state, and belong to two separate subgenera making a possibly interesting comparison between the two. We will aim to collect 20 workers from both species with a minimum of 15 at each site (N=40). Individuals will be transported in coolers and stored in -20° freezers until they are transported back to the Jamieson Lab. Pathogen screening will later investigate infection of Crithidia bombi, Variamorpha bombi, and conopid fly (Conopidae, Diptera) parasitism through microscopy and molecular analysis.