Methods:
I will perform a crossbreeding experiment of E. hageni to determine the genetics of this species’ polymorphism. My advising professor, Dr. Ola Fincke, has indicated that the University of Michigan Biological Station’s properties on Sugar Island, MI, contains a nice, stable population of E. hageni. I will capture males and females of E. hageni. Within insectaries that I have built, I will mate one male with a heteromorph and andromorph. I will use at least 5 males to get the offspring of 10 females. After mating, the females will oviposit in a plastic jar with moist filter paper (over 1-3 days ). A female lays 100-300 eggs. After about a week, the larvae hatch (pers. obs.) and I will transfer them to rearing containers and feed them brine shrimp throughout the larval stage. Since the larval stage occurs over several months, rearing will take place in part in Michigan and then be transferred to my lab at the University of Oklahoma. Once the nymphs emerge as tenerals, they will be transferred to a humid environment to mature. When adult color is fixed, I will mate these adults to each other to attain an F2 generation, if possible.