Feeding ecology will be assessed through creel surveys on Burt and Mullet Lakes, quantifying lamprey host selection via wounding reported by fishers. Fatty acid analyses will also be conducted on previously obtained lamprey to determine their feeding habits. Sea lamprey embryo viability will be determined through spawning nest surveys within the Maple, Pigeon and Sturgeon Rivers. Finally, morphometric measurements and analyses of stable isotopes, statolith microchemistry, and fatty acids will be utilized to distinguish landlocked sea lamprey from those which feed in Lake Huron.