Methods:
This experiment will examine potential differences in the frequency and duration of urine release for males and females in one of three treatments (male-male, female-female, male-female contests) using the injection of fluorescein dye and black lights as well as a specialized fight arena that allows video recording from above and below the experiment. The two contestants engaged in the contest will each be given a different dye color in order for us to also characterize plume shape and size at different time points in the contest. Animals will be collected from Maple Bay (Burt Lake) and will be housed in Lakeside Lab in flow through troughs. All animals will be frozen at the conclusion of the experiment.