This dataset corresponds to 5,500 single-particle, dual-polarity mass spectra of biomass burning particles measured from a mobile laboratory during the 2017 prescribed burn at the University of Michigan Biological Station near Pellston, Michigan. Size-resolved chemical composition of individual particles, primarily ranging from 0.15 - 0.60 μm in vacuum aerodynamic diameter, was measured using an aerosol time-of-flight mass spectrometer (ATOFMS).