Methods:
This study will take place in a northern temperate forest at the University of Michigan Biological Station forest. The Forest Accelerated Succession Experiment (FASET) manipulation caused an increased rate of forest succession by stem girdling early-successional species (birch, and aspen), causing these relatively short-lived species to die in place and prompting re-allocation of limiting resources to later-successional species such as oaks and maples.
I will quantify light with a LAI 2000 leaf area analyzer (LI-COR Biosciences). I will collect soil solutions from zero tension lysimeters (ZTL) weekly to assess available N losses and soil solution nutrient content. Quantification of N will come from lysimeter solutions and measure NH4+ and NO3-. Ammonium and nitrate are determined colormetrically via Bran-Leubbe or Smartchem analyzers. I will repeat a census of seedlings and compare changes in height, leaf number, stem thickness, understory plant cover and pathogen damage between treatment and controls. I will collect census data from three 1x1 meter quadrats in eight, 16 meter diameter plots distributed in treated and control areas. I will examine how changes in the plants are correlated with light and N. I plan to test the hypotheses by comparing composition and structure of understory plants in treatment and control plots.