Methods:
Physella acuta snails are collected in Douglas Lake. I collect them by snorkeling, picking them up with my hands and placing them in a bucket I have with me, and then I walk them to my space in the boat well. They are stored in a holding tank until they are moved to a treatment tank of either increased heat, lowered pH or both. Crayfish, a natural predator of the snails, were collected at the beginning of the study; they are kept in individual tanks. Their purpose is to contaminate their tank water to contain chemical cues of crayfish to use to trigger the antipredator response in snails during experimentation. Each day, I take the pH and temperature of all tanks. The snails stay in their treatment tanks for 3 days before experimental trials. Then they are placed into separate 600mL beakers, containing 100mL crayfish water and 200mL of their treatment water. I time how long it takes for them to perform an antipredator response. After experimentation, the snails are placed into a secondary holding tank. They are held there until the end of the study; we keep them for the duration of the study to avoid potential recapture.