Methods:
We will collect 5 ramets (all above ground parts + roots and rhizome section) of native and invasive Phragmites from each of 7 sites in northern Michigan where both lineages currently coexist. Sites are managed by the Little Traverse Land Conservancy and Tip of the Mitt Watershed Council. Soil samples will also be collected from each site.
Plant tissues will be washed and surface sterilized in preparation for genetic analyses. Root sections will be cleared and stained for fungal examination. Soils will be analyzed for plant available nutrients (N & P).